m5 cytokine mixture  (PeproTech)

Journal: Molecular Medicine

Article Title: Paris saponin VII attenuates psoriasiform inflammation by regulating STAT3/NFκB signaling pathway and Caspase-1-induced pyroptosis

doi: 10.1186/s10020-025-01253-y

Figure Lengend Snippet: PSVII exhibited anti-inflammatory and antioxidant effects in IMQ-induced psoriasis-like mice. ( A ) ELISA analysis of serum levels of IL-17, IL-23, IL-2, IL-6 and TNF-α. ( B ) and ( C ) Relative expression of TNF-α in PSVII -treated psoriasis models in vitro. ( D ) Immunofluorescence microscopy of HaCaT sections treated with PSVII , probing for TNF-α infiltration (scale bar = 10 μm). ( E ) ROS expression in various groups, as observed under fluorescence microscopy (scale bar = 20 μm). n = 6 mice/group, n = 3 cells/group. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Model group

Article Snippet: The cells were divided into the following groups: (Griffiths et al. ) Control group; (Chandran ) Model group; and (Lee and Kim ) PSVII treatment groups, which received PSVII at concentrations of 2 μM, 5 μM, and 10 μM (designated as PSVII -L, PSVII -M, and PSVII -H, respectively), for 24 h. A psoriasis-like model was induced in the model group using M5 (The cytokine mixture composed of IL-17, IL-22, IL-1α, TNF-α, and Oncostatin M, the concentration was 10 ng/ml, Novoprotein, China), as previously described (Zhao et al. ).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, In Vitro, Immunofluorescence, Microscopy, Fluorescence